Construct: shRNA TRCN0000021845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005206.3-715s1c1
DNA Barcode:
CGCCTCAGTATCGGCTCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRK (1398)
Gene Description:
CRK proto-oncogene, adaptor protein
Transcript:
RefSeq NM_005206.3 (NON-CURRENT)
Match location:
Position 715 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTCAGTATCGGCTCTGAT
Hairpin Sequence:
5'-CCGG-CGCCTCAGTATCGGCTCTGAT-CTCGAG-ATCAGAGCCGATACTGAGGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTCAGTATCGGCTCTGATCTCGAGATCAGAGCCGATACTGAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTCAGTATCGGCTCTGATCTCGAGATCAGAGCCGATACTGAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000281380