Construct: shRNA TRCN0000021871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006234.3-357s1c1
DNA Barcode:
GAGCGCTTTCGGGTGGCCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLR2J (5439)
Gene Description:
RNA polymerase II subunit J
Transcript:
RefSeq NM_006234.3 (NON-CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGCTTTCGGGTGGCCATA
Hairpin Sequence:
5'-CCGG-GAGCGCTTTCGGGTGGCCATA-CTCGAG-TATGGCCACCCGAAAGCGCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGCTTTCGGGTGGCCATACTCGAGTATGGCCACCCGAAAGCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGCTTTCGGGTGGCCATACTCGAGTATGGCCACCCGAAAGCGCTC-3'

Other clones with same target sequence:

(none)