Construct: shRNA TRCN0000021975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005935.1-3282s1c1
DNA Barcode:
GCCTCAAGTGAAGTTTGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AFF1 (4299)
Gene Description:
AF4/FMR2 family member 1
Transcript:
RefSeq NM_005935.1 (NON-CURRENT)
Match location:
Position 3282 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAAGTGAAGTTTGACAA
Hairpin Sequence:
5'-CCGG-GCCTCAAGTGAAGTTTGACAA-CTCGAG-TTGTCAAACTTCACTTGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAAGTGAAGTTTGACAACTCGAGTTGTCAAACTTCACTTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAAGTGAAGTTTGACAACTCGAGTTGTCAAACTTCACTTGAGGC-3'

Other clones with same target sequence:

  1. TRCN0000330907