Construct: shRNA TRCN0000021981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021252.3-435s1c1
DNA Barcode:
CGTGAAGTCGATAGAAATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB18 (22931)
Gene Description:
RAB18, member RAS oncogene family
Transcript:
RefSeq NM_021252.3 (NON-CURRENT)
Match location:
Position 435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000021981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAAGTCGATAGAAATGAA
Hairpin Sequence:
5'-CCGG-CGTGAAGTCGATAGAAATGAA-CTCGAG-TTCATTTCTATCGACTTCACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAAGTCGATAGAAATGAACTCGAGTTCATTTCTATCGACTTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAAGTCGATAGAAATGAACTCGAGTTCATTTCTATCGACTTCACG-3'

Other clones with same target sequence:

(none)