Construct: shRNA TRCN0000022024

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004269.2-639s1c1
DNA Barcode:
GCCTGTTCATTGATCGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MED27 (9442)
Gene Description:
mediator complex subunit 27
Transcript:
RefSeq NM_004269.2 (NON-CURRENT)
Match location:
Position 639 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022024)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTTCATTGATCGAACAA
Hairpin Sequence:
5'-CCGG-GCCTGTTCATTGATCGAACAA-CTCGAG-TTGTTCGATCAATGAACAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTTCATTGATCGAACAACTCGAGTTGTTCGATCAATGAACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTTCATTGATCGAACAACTCGAGTTGTTCGATCAATGAACAGGC-3'

Other clones with same target sequence:

  1. TRCN0000330483