Construct: shRNA TRCN0000022111

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005119.1-1226s1c1
DNA Barcode:
GCAGCCTATACAAAGAGGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THRAP3 (9967)
Gene Description:
thyroid hormone receptor associated protein 3
Transcript:
RefSeq NM_005119.1 (NON-CURRENT)
Match location:
Position 1226 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022111)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCTATACAAAGAGGTAT
Hairpin Sequence:
5'-CCGG-GCAGCCTATACAAAGAGGTAT-CTCGAG-ATACCTCTTTGTATAGGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCTATACAAAGAGGTATCTCGAGATACCTCTTTGTATAGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCTATACAAAGAGGTATCTCGAGATACCTCTTTGTATAGGCTGC-3'

Other clones with same target sequence:

(none)