Construct: shRNA TRCN0000022123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002518.2-2123s1c1
DNA Barcode:
CCAATGAGAAGCTCACAGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPAS2 (4862)
Gene Description:
neuronal PAS domain protein 2
Transcript:
RefSeq NM_002518.2 (NON-CURRENT)
Match location:
Position 2123 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATGAGAAGCTCACAGCTA
Hairpin Sequence:
5'-CCGG-CCAATGAGAAGCTCACAGCTA-CTCGAG-TAGCTGTGAGCTTCTCATTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATGAGAAGCTCACAGCTACTCGAGTAGCTGTGAGCTTCTCATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATGAGAAGCTCACAGCTACTCGAGTAGCTGTGAGCTTCTCATTGG-3'

Other clones with same target sequence:

(none)