Construct: shRNA TRCN0000022196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007344.2-1414s1c1
DNA Barcode:
GCCAAGGTTAGAACCTGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTF1 (7270)
Gene Description:
transcription termination factor 1
Transcript:
RefSeq NM_007344.2 (NON-CURRENT)
Match location:
Position 1414 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGGTTAGAACCTGCAAA
Hairpin Sequence:
5'-CCGG-GCCAAGGTTAGAACCTGCAAA-CTCGAG-TTTGCAGGTTCTAACCTTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGGTTAGAACCTGCAAACTCGAGTTTGCAGGTTCTAACCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGGTTAGAACCTGCAAACTCGAGTTTGCAGGTTCTAACCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000275519