Construct: shRNA TRCN0000022273

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007034.3-200s1c1
DNA Barcode:
GAGCTTCAGATGAAGATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJB4 (11080)
Gene Description:
DnaJ heat shock protein family (Hsp40) member B4
Transcript:
RefSeq NM_007034.3 (NON-CURRENT)
Match location:
Position 200 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022273)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTTCAGATGAAGATATTA
Hairpin Sequence:
5'-CCGG-GAGCTTCAGATGAAGATATTA-CTCGAG-TAATATCTTCATCTGAAGCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTTCAGATGAAGATATTACTCGAGTAATATCTTCATCTGAAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTTCAGATGAAGATATTACTCGAGTAATATCTTCATCTGAAGCTC-3'

Other clones with same target sequence:

(none)