Construct: shRNA TRCN0000022286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014280.1-356s1c1
DNA Barcode:
GCTTACAAGTTGCTACTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJC8 (22826)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C8
Transcript:
RefSeq NM_014280.1 (NON-CURRENT)
Match location:
Position 356 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTACAAGTTGCTACTGGAT
Hairpin Sequence:
5'-CCGG-GCTTACAAGTTGCTACTGGAT-CTCGAG-ATCCAGTAGCAACTTGTAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTACAAGTTGCTACTGGATCTCGAGATCCAGTAGCAACTTGTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTACAAGTTGCTACTGGATCTCGAGATCCAGTAGCAACTTGTAAGC-3'

Other clones with same target sequence:

  1. TRCN0000342806