Construct: shRNA TRCN0000022325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080732.1-1174s1c1
DNA Barcode:
GCTGCATCACCTGTATCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGLN2 (112398)
Gene Description:
egl-9 family hypoxia inducible factor 2
Transcript:
RefSeq NM_080732.1 (NON-CURRENT)
Match location:
Position 1174 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCATCACCTGTATCTATT
Hairpin Sequence:
5'-CCGG-GCTGCATCACCTGTATCTATT-CTCGAG-AATAGATACAGGTGATGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCATCACCTGTATCTATTCTCGAGAATAGATACAGGTGATGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCATCACCTGTATCTATTCTCGAGAATAGATACAGGTGATGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000318672