Construct: shRNA TRCN0000022342

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_085225.1-312s1c1
DNA Barcode:
TGCTGTGTGTGAAGCACTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC145760 (145760)
Gene Description:
hypothetical LOC145760
Transcript:
RefSeq XM_085225.1 (NON-CURRENT)
Match location:
Position 312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022342)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGTGTGTGAAGCACTCAA
Hairpin Sequence:
5'-CCGG-TGCTGTGTGTGAAGCACTCAA-CTCGAG-TTGAGTGCTTCACACACAGCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGTGTGTGAAGCACTCAACTCGAGTTGAGTGCTTCACACACAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGTGTGTGAAGCACTCAACTCGAGTTGAGTGCTTCACACACAGCA-3'

Other clones with same target sequence:

(none)