Construct: shRNA TRCN0000022374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004238.1-6162s1c1
DNA Barcode:
CCTGAGTCAAGGAAACATGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIP12 (9320)
Gene Description:
thyroid hormone receptor interactor 12
Transcript:
RefSeq NM_004238.1 (NON-CURRENT)
Match location:
Position 6162 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGTCAAGGAAACATGTT
Hairpin Sequence:
5'-CCGG-CCTGAGTCAAGGAAACATGTT-CTCGAG-AACATGTTTCCTTGACTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGTCAAGGAAACATGTTCTCGAGAACATGTTTCCTTGACTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGTCAAGGAAACATGTTCTCGAGAACATGTTTCCTTGACTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000273135