Construct: shRNA TRCN0000022397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017876.3-1064s1c1
DNA Barcode:
CCTCTGTCTAACCTCACCCTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF126 (55658)
Gene Description:
ring finger protein 126
Transcript:
RefSeq NM_017876.3 (NON-CURRENT)
Match location:
Position 1064 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGTCTAACCTCACCCTC
Hairpin Sequence:
5'-CCGG-CCTCTGTCTAACCTCACCCTC-CTCGAG-GAGGGTGAGGTTAGACAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGTCTAACCTCACCCTCCTCGAGGAGGGTGAGGTTAGACAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGTCTAACCTCACCCTCCTCGAGGAGGGTGAGGTTAGACAGAGG-3'

Other clones with same target sequence:

(none)