Construct: shRNA TRCN0000022404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023079.2-2212s1c1
DNA Barcode:
CCTGGCTTTGTTGCTTCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2Z (65264)
Gene Description:
ubiquitin conjugating enzyme E2 Z
Transcript:
RefSeq NM_023079.2 (NON-CURRENT)
Match location:
Position 2212 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCTTTGTTGCTTCTCTA
Hairpin Sequence:
5'-CCGG-CCTGGCTTTGTTGCTTCTCTA-CTCGAG-TAGAGAAGCAACAAAGCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCTTTGTTGCTTCTCTACTCGAGTAGAGAAGCAACAAAGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCTTTGTTGCTTCTCTACTCGAGTAGAGAAGCAACAAAGCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000320605