Construct: shRNA TRCN0000022428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024778.3-1779s1c1
DNA Barcode:
GCCTGATGATTCGTAGATGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LONRF3 (79836)
Gene Description:
LON peptidase N-terminal domain and ring finger 3
Transcript:
RefSeq NM_024778.3 (NON-CURRENT)
Match location:
Position 1779 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGATGATTCGTAGATGCA
Hairpin Sequence:
5'-CCGG-GCCTGATGATTCGTAGATGCA-CTCGAG-TGCATCTACGAATCATCAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGATGATTCGTAGATGCACTCGAGTGCATCTACGAATCATCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGATGATTCGTAGATGCACTCGAGTGCATCTACGAATCATCAGGC-3'

Other clones with same target sequence:

(none)