Construct: shRNA TRCN0000022438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024907.5-803s1c1
DNA Barcode:
GTGGTCAAGTTCTCAGCCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO17 (115290)
Gene Description:
F-box protein 17
Transcript:
RefSeq NM_024907.5 (NON-CURRENT)
Match location:
Position 803 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTCAAGTTCTCAGCCTCA
Hairpin Sequence:
5'-CCGG-GTGGTCAAGTTCTCAGCCTCA-CTCGAG-TGAGGCTGAGAACTTGACCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTCAAGTTCTCAGCCTCACTCGAGTGAGGCTGAGAACTTGACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTCAAGTTCTCAGCCTCACTCGAGTGAGGCTGAGAACTTGACCAC-3'

Other clones with same target sequence:

  1. TRCN0000342497