Construct: shRNA TRCN0000022455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028679.2-571s1c1
DNA Barcode:
CCCTATCAGCTTCCAGAGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Irak3 (73914)
Gene Description:
interleukin-1 receptor-associated kinase 3
Transcript:
RefSeq NM_028679.2 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATCAGCTTCCAGAGTAT
Hairpin Sequence:
5'-CCGG-CCCTATCAGCTTCCAGAGTAT-CTCGAG-ATACTCTGGAAGCTGATAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATCAGCTTCCAGAGTATCTCGAGATACTCTGGAAGCTGATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATCAGCTTCCAGAGTATCTCGAGATACTCTGGAAGCTGATAGGG-3'

Other clones with same target sequence:

(none)