Construct: shRNA TRCN0000022480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009370.2-1394s1c1
DNA Barcode:
GCCTTGAGAGTGATGGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tgfbr1 (21812)
Gene Description:
transforming growth factor, beta receptor I
Transcript:
RefSeq NM_009370.2 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGAGAGTGATGGCTAAA
Hairpin Sequence:
5'-CCGG-GCCTTGAGAGTGATGGCTAAA-CTCGAG-TTTAGCCATCACTCTCAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGAGAGTGATGGCTAAACTCGAGTTTAGCCATCACTCTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGAGAGTGATGGCTAAACTCGAGTTTAGCCATCACTCTCAAGGC-3'

Other clones with same target sequence:

(none)