Construct: shRNA TRCN0000022498

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007560.3-1240s1c1
DNA Barcode:
GCAAAGTCCATGCTGAAGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmpr1b (12167)
Gene Description:
bone morphogenetic protein receptor, type 1B
Transcript:
RefSeq NM_007560.3 (NON-CURRENT)
Match location:
Position 1240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022498)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGTCCATGCTGAAGCTA
Hairpin Sequence:
5'-CCGG-GCAAAGTCCATGCTGAAGCTA-CTCGAG-TAGCTTCAGCATGGACTTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGTCCATGCTGAAGCTACTCGAGTAGCTTCAGCATGGACTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGTCCATGCTGAAGCTACTCGAGTAGCTTCAGCATGGACTTTGC-3'

Other clones with same target sequence:

(none)