Construct: shRNA TRCN0000022500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172161.2-1589s1c1
DNA Barcode:
CCACAGGCTCATCTTCCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Irak2 (108960)
Gene Description:
interleukin-1 receptor-associated kinase 2
Transcript:
RefSeq NM_172161.2 (NON-CURRENT)
Match location:
Position 1589 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGGCTCATCTTCCAATA
Hairpin Sequence:
5'-CCGG-CCACAGGCTCATCTTCCAATA-CTCGAG-TATTGGAAGATGAGCCTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGGCTCATCTTCCAATACTCGAGTATTGGAAGATGAGCCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGGCTCATCTTCCAATACTCGAGTATTGGAAGATGAGCCTGTGG-3'

Other clones with same target sequence:

(none)