Construct: shRNA TRCN0000022522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007394.2-1230s1c1
DNA Barcode:
CAGAGCACAAACCAGCTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acvr1 (11477)
Gene Description:
activin A receptor, type 1
Transcript:
RefSeq NM_007394.2 (NON-CURRENT)
Match location:
Position 1230 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGCACAAACCAGCTTGAT
Hairpin Sequence:
5'-CCGG-CAGAGCACAAACCAGCTTGAT-CTCGAG-ATCAAGCTGGTTTGTGCTCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGCACAAACCAGCTTGATCTCGAGATCAAGCTGGTTTGTGCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGCACAAACCAGCTTGATCTCGAGATCAAGCTGGTTTGTGCTCTG-3'

Other clones with same target sequence:

(none)