Construct: shRNA TRCN0000022548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010717.1-662s1c1
DNA Barcode:
TGTGGAAACTTCATTGGCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Limk1 (16885)
Gene Description:
LIM-domain containing, protein kinase
Transcript:
RefSeq NM_010717.1 (NON-CURRENT)
Match location:
Position 662 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGAAACTTCATTGGCGAT
Hairpin Sequence:
5'-CCGG-TGTGGAAACTTCATTGGCGAT-CTCGAG-ATCGCCAATGAAGTTTCCACA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGAAACTTCATTGGCGATCTCGAGATCGCCAATGAAGTTTCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGAAACTTCATTGGCGATCTCGAGATCGCCAATGAAGTTTCCACA-3'

Other clones with same target sequence:

  1. TRCN0000345039