Construct: shRNA TRCN0000022559

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172688.1-1209s1c1
DNA Barcode:
AGGCAAAGCAACAGAGTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map3k7 (26409)
Gene Description:
mitogen-activated protein kinase kinase kinase 7
Transcript:
RefSeq NM_172688.1 (NON-CURRENT)
Match location:
Position 1209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022559)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCAAAGCAACAGAGTGAAT
Hairpin Sequence:
5'-CCGG-AGGCAAAGCAACAGAGTGAAT-CTCGAG-ATTCACTCTGTTGCTTTGCCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCAAAGCAACAGAGTGAATCTCGAGATTCACTCTGTTGCTTTGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCAAAGCAACAGAGTGAATCTCGAGATTCACTCTGTTGCTTTGCCT-3'

Other clones with same target sequence:

  1. TRCN0000322277