Construct: shRNA TRCN0000022607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_194020.3-1320s1c1
DNA Barcode:
CCAGTTGCCTTACTATGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acvr1c (269275)
Gene Description:
activin A receptor, type IC
Transcript:
RefSeq XM_194020.3 (NON-CURRENT)
Match location:
Position 1320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTGCCTTACTATGACAT
Hairpin Sequence:
5'-CCGG-CCAGTTGCCTTACTATGACAT-CTCGAG-ATGTCATAGTAAGGCAACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTGCCTTACTATGACATCTCGAGATGTCATAGTAAGGCAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTGCCTTACTATGACATCTCGAGATGTCATAGTAAGGCAACTGG-3'

Other clones with same target sequence:

(none)