Construct: shRNA TRCN0000022631

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_357543.1-297s1c1
DNA Barcode:
CAAACCCTTGAACCTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm1390 (384284)
Gene Description:
gene model 1390, (NCBI)
Transcript:
RefSeq XM_357543.1 (NON-CURRENT)
Match location:
Position 297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022631)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACCCTTGAACCTCAAGAT
Hairpin Sequence:
5'-CCGG-CAAACCCTTGAACCTCAAGAT-CTCGAG-ATCTTGAGGTTCAAGGGTTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACCCTTGAACCTCAAGATCTCGAGATCTTGAGGTTCAAGGGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACCCTTGAACCTCAAGATCTCGAGATCTTGAGGTTCAAGGGTTTG-3'

Other clones with same target sequence:

(none)