Construct: shRNA TRCN0000022641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007397.1-1336s1c1
DNA Barcode:
CGATGAGTACATGCTGCCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acvr2b (11481)
Gene Description:
activin receptor IIB
Transcript:
RefSeq NM_007397.1 (NON-CURRENT)
Match location:
Position 1336 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGAGTACATGCTGCCCTT
Hairpin Sequence:
5'-CCGG-CGATGAGTACATGCTGCCCTT-CTCGAG-AAGGGCAGCATGTACTCATCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGAGTACATGCTGCCCTTCTCGAGAAGGGCAGCATGTACTCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGAGTACATGCTGCCCTTCTCGAGAAGGGCAGCATGTACTCATCG-3'

Other clones with same target sequence:

(none)