Construct: shRNA TRCN0000022666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_124895.3-822s1c1
DNA Barcode:
CGACCAGATTTACGCCATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC233024 (233024)
Gene Description:
similar to protein kinase C zeta
Transcript:
RefSeq XM_124895.3 (NON-CURRENT)
Match location:
Position 822 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCAGATTTACGCCATGAA
Hairpin Sequence:
5'-CCGG-CGACCAGATTTACGCCATGAA-CTCGAG-TTCATGGCGTAAATCTGGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCAGATTTACGCCATGAACTCGAGTTCATGGCGTAAATCTGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCAGATTTACGCCATGAACTCGAGTTCATGGCGTAAATCTGGTCG-3'

Other clones with same target sequence:

(none)