Construct: shRNA TRCN0000022673

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007434.2-391s1c1
DNA Barcode:
CGACCCAACACCTTTGTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akt2 (11652)
Gene Description:
thymoma viral proto-oncogene 2
Transcript:
RefSeq NM_007434.2 (NON-CURRENT)
Match location:
Position 391 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022673)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCCAACACCTTTGTCATA
Hairpin Sequence:
5'-CCGG-CGACCCAACACCTTTGTCATA-CTCGAG-TATGACAAAGGTGTTGGGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCCAACACCTTTGTCATACTCGAGTATGACAAAGGTGTTGGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCCAACACCTTTGTCATACTCGAGTATGACAAAGGTGTTGGGTCG-3'

Other clones with same target sequence:

  1. TRCN0000310882