Construct: shRNA TRCN0000022685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011102.2-842s1c1
DNA Barcode:
CGTCGGATGAGATCCATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkcg (18752)
Gene Description:
protein kinase C, gamma
Transcript:
RefSeq NM_011102.2 (NON-CURRENT)
Match location:
Position 842 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCGGATGAGATCCATATTA
Hairpin Sequence:
5'-CCGG-CGTCGGATGAGATCCATATTA-CTCGAG-TAATATGGATCTCATCCGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCGGATGAGATCCATATTACTCGAGTAATATGGATCTCATCCGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCGGATGAGATCCATATTACTCGAGTAATATGGATCTCATCCGACG-3'

Other clones with same target sequence:

(none)