Construct: shRNA TRCN0000022691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183016.1-3573s1c1
DNA Barcode:
CCTCAGATGTTATCCATGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc42bpb (217866)
Gene Description:
CDC42 binding protein kinase beta
Transcript:
RefSeq NM_183016.1 (NON-CURRENT)
Match location:
Position 3573 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGATGTTATCCATGCTA
Hairpin Sequence:
5'-CCGG-CCTCAGATGTTATCCATGCTA-CTCGAG-TAGCATGGATAACATCTGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGATGTTATCCATGCTACTCGAGTAGCATGGATAACATCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGATGTTATCCATGCTACTCGAGTAGCATGGATAACATCTGAGG-3'

Other clones with same target sequence:

(none)