Construct: shRNA TRCN0000022692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183016.1-2215s1c1
DNA Barcode:
GCTAGAAGTAAAGAACGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc42bpb (217866)
Gene Description:
CDC42 binding protein kinase beta
Transcript:
RefSeq NM_183016.1 (NON-CURRENT)
Match location:
Position 2215 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGAAGTAAAGAACGTGAA
Hairpin Sequence:
5'-CCGG-GCTAGAAGTAAAGAACGTGAA-CTCGAG-TTCACGTTCTTTACTTCTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGAAGTAAAGAACGTGAACTCGAGTTCACGTTCTTTACTTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGAAGTAAAGAACGTGAACTCGAGTTCACGTTCTTTACTTCTAGC-3'

Other clones with same target sequence:

(none)