Construct: shRNA TRCN0000022711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011299.3-627s1c1
DNA Barcode:
GCTTTCCAAAGAGGTGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps6ka2 (20112)
Gene Description:
ribosomal protein S6 kinase, polypeptide 2
Transcript:
RefSeq NM_011299.3 (NON-CURRENT)
Match location:
Position 627 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCCAAAGAGGTGATGTT
Hairpin Sequence:
5'-CCGG-GCTTTCCAAAGAGGTGATGTT-CTCGAG-AACATCACCTCTTTGGAAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCCAAAGAGGTGATGTTCTCGAGAACATCACCTCTTTGGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCCAAAGAGGTGATGTTCTCGAGAACATCACCTCTTTGGAAAGC-3'

Other clones with same target sequence:

(none)