Construct: shRNA TRCN0000022729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207246.2-573s1c1
DNA Barcode:
GCAGAGTTTAATTTGGATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rasgrp3 (240168)
Gene Description:
RAS, guanyl releasing protein 3
Transcript:
RefSeq NM_207246.2 (NON-CURRENT)
Match location:
Position 573 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTTTAATTTGGATCTT
Hairpin Sequence:
5'-CCGG-GCAGAGTTTAATTTGGATCTT-CTCGAG-AAGATCCAAATTAAACTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTTTAATTTGGATCTTCTCGAGAAGATCCAAATTAAACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTTTAATTTGGATCTTCTCGAGAAGATCCAAATTAAACTCTGC-3'

Other clones with same target sequence:

(none)