Construct: shRNA TRCN0000022745

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133220.1-441s1c1
DNA Barcode:
CGCAGAGTTTGACAAACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgk3 (170755)
Gene Description:
serum/glucocorticoid regulated kinase 3
Transcript:
RefSeq NM_133220.1 (NON-CURRENT)
Match location:
Position 441 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022745)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGAGTTTGACAAACTTTA
Hairpin Sequence:
5'-CCGG-CGCAGAGTTTGACAAACTTTA-CTCGAG-TAAAGTTTGTCAAACTCTGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGAGTTTGACAAACTTTACTCGAGTAAAGTTTGTCAAACTCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGAGTTTGACAAACTTTACTCGAGTAAAGTTTGTCAAACTCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000298002