Construct: shRNA TRCN0000022751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007708.1-1459s1c1
DNA Barcode:
GCCGCTAAGATGAATTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cit (12704)
Gene Description:
citron
Transcript:
RefSeq NM_007708.1 (NON-CURRENT)
Match location:
Position 1459 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCTAAGATGAATTCAAAT
Hairpin Sequence:
5'-CCGG-GCCGCTAAGATGAATTCAAAT-CTCGAG-ATTTGAATTCATCTTAGCGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCTAAGATGAATTCAAATCTCGAGATTTGAATTCATCTTAGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCTAAGATGAATTCAAATCTCGAGATTTGAATTCATCTTAGCGGC-3'

Other clones with same target sequence:

  1. TRCN0000321774