Construct: shRNA TRCN0000022762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011104.1-340s1c1
DNA Barcode:
CGTCACTTCGAGGACTGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkce (18754)
Gene Description:
protein kinase C, epsilon
Transcript:
RefSeq NM_011104.1 (NON-CURRENT)
Match location:
Position 340 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCACTTCGAGGACTGGATT
Hairpin Sequence:
5'-CCGG-CGTCACTTCGAGGACTGGATT-CTCGAG-AATCCAGTCCTCGAAGTGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCACTTCGAGGACTGGATTCTCGAGAATCCAGTCCTCGAAGTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCACTTCGAGGACTGGATTCTCGAGAATCCAGTCCTCGAAGTGACG-3'

Other clones with same target sequence:

(none)