Construct: shRNA TRCN0000022785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016979.1-992s1c1
DNA Barcode:
CCGTTTGGGATTTACCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkx (19108)
Gene Description:
protein kinase, X-linked
Transcript:
RefSeq NM_016979.1 (CURRENT)
Match location:
Position 992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTTGGGATTTACCAGAAA
Hairpin Sequence:
5'-CCGG-CCGTTTGGGATTTACCAGAAA-CTCGAG-TTTCTGGTAAATCCCAAACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTTGGGATTTACCAGAAACTCGAGTTTCTGGTAAATCCCAAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTTGGGATTTACCAGAAACTCGAGTTTCTGGTAAATCCCAAACGG-3'

Other clones with same target sequence:

(none)