Construct: shRNA TRCN0000022821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178749.2-351s1c1
DNA Barcode:
GTCTGCATTGTGCGGAAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk32a (269019)
Gene Description:
serine/threonine kinase 32A
Transcript:
RefSeq NM_178749.2 (NON-CURRENT)
Match location:
Position 351 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGCATTGTGCGGAAGAAT
Hairpin Sequence:
5'-CCGG-GTCTGCATTGTGCGGAAGAAT-CTCGAG-ATTCTTCCGCACAATGCAGAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGCATTGTGCGGAAGAATCTCGAGATTCTTCCGCACAATGCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGCATTGTGCGGAAGAATCTCGAGATTCTTCCGCACAATGCAGAC-3'

Other clones with same target sequence:

(none)