Construct: shRNA TRCN0000022826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009097.1-685s1c1
DNA Barcode:
GCACAGCTTGGGCATTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps6ka1 (20111)
Gene Description:
ribosomal protein S6 kinase polypeptide 1
Transcript:
RefSeq NM_009097.1 (NON-CURRENT)
Match location:
Position 685 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGCTTGGGCATTATTTA
Hairpin Sequence:
5'-CCGG-GCACAGCTTGGGCATTATTTA-CTCGAG-TAAATAATGCCCAAGCTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGCTTGGGCATTATTTACTCGAGTAAATAATGCCCAAGCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGCTTGGGCATTATTTACTCGAGTAAATAATGCCCAAGCTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000285730