Construct: shRNA TRCN0000022829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018869.2-353s1c1
DNA Barcode:
CCTGGACTTAGTGGCAGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grk5 (14773)
Gene Description:
G protein-coupled receptor kinase 5
Transcript:
RefSeq NM_018869.2 (NON-CURRENT)
Match location:
Position 353 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGACTTAGTGGCAGAATA
Hairpin Sequence:
5'-CCGG-CCTGGACTTAGTGGCAGAATA-CTCGAG-TATTCTGCCACTAAGTCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGACTTAGTGGCAGAATACTCGAGTATTCTGCCACTAAGTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGACTTAGTGGCAGAATACTCGAGTATTCTGCCACTAAGTCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000285245