Construct: shRNA TRCN0000022902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009071.1-3844s1c1
DNA Barcode:
CCGTGCAAAGTAAGTTACGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rock1 (19877)
Gene Description:
Rho-associated coiled-coil containing protein kinase 1
Transcript:
RefSeq NM_009071.1 (NON-CURRENT)
Match location:
Position 3844 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGCAAAGTAAGTTACGAT
Hairpin Sequence:
5'-CCGG-CCGTGCAAAGTAAGTTACGAT-CTCGAG-ATCGTAACTTACTTTGCACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGCAAAGTAAGTTACGATCTCGAGATCGTAACTTACTTTGCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGCAAAGTAAGTTACGATCTCGAGATCGTAACTTACTTTGCACGG-3'

Other clones with same target sequence:

(none)