Construct: shRNA TRCN0000022938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009652.1-1033s1c1
DNA Barcode:
CCGCTTCTATGGTGCGGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akt1 (11651)
Gene Description:
thymoma viral proto-oncogene 1
Transcript:
RefSeq NM_009652.1 (NON-CURRENT)
Match location:
Position 1033 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTTCTATGGTGCGGAGAT
Hairpin Sequence:
5'-CCGG-CCGCTTCTATGGTGCGGAGAT-CTCGAG-ATCTCCGCACCATAGAAGCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTTCTATGGTGCGGAGATCTCGAGATCTCCGCACCATAGAAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTTCTATGGTGCGGAGATCTCGAGATCTCCGCACCATAGAAGCGG-3'

Other clones with same target sequence:

(none)