Construct: shRNA TRCN0000022940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_194716.2-202s1c1
DNA Barcode:
CCACCCAAATTTGGCACACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lats1 (16798)
Gene Description:
large tumor suppressor
Transcript:
RefSeq XM_194716.2 (NON-CURRENT)
Match location:
Position 202 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCCAAATTTGGCACACAT
Hairpin Sequence:
5'-CCGG-CCACCCAAATTTGGCACACAT-CTCGAG-ATGTGTGCCAAATTTGGGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCCAAATTTGGCACACATCTCGAGATGTGTGCCAAATTTGGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCCAAATTTGGCACACATCTCGAGATGTGTGCCAAATTTGGGTGG-3'

Other clones with same target sequence:

(none)