Construct: shRNA TRCN0000022979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355257.1-350s1c1
DNA Barcode:
CCAGGATGACAATAACTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381309 (381309)
Gene Description:
similar to CDC42-binding protein kinase alpha; mytonic dystrophy kinase-related Cdc42-binding kinase
Transcript:
RefSeq XM_355257.1 (NON-CURRENT)
Match location:
Position 350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGATGACAATAACTTATA
Hairpin Sequence:
5'-CCGG-CCAGGATGACAATAACTTATA-CTCGAG-TATAAGTTATTGTCATCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGATGACAATAACTTATACTCGAGTATAAGTTATTGTCATCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGATGACAATAACTTATACTCGAGTATAAGTTATTGTCATCCTGG-3'

Other clones with same target sequence:

(none)