Construct: shRNA TRCN0000022998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355003.1-773s1c1
DNA Barcode:
CCACGTTCAGTTTCTCATCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381082 (381082)
Gene Description:
similar to mitogen-activated protein kinase 14 isoform 1; cytokine suppressive anti-inflammatory drug binding protein; Csaids binding protein; MAP kinase Mxi2; p38 mitogen activated protein kinase; p38 MAP kinase; p38alpha Exip; stress-activated pr...
Transcript:
RefSeq XM_355003.1 (NON-CURRENT)
Match location:
Position 773 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000022998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGTTCAGTTTCTCATCTA
Hairpin Sequence:
5'-CCGG-CCACGTTCAGTTTCTCATCTA-CTCGAG-TAGATGAGAAACTGAACGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGTTCAGTTTCTCATCTACTCGAGTAGATGAGAAACTGAACGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGTTCAGTTTCTCATCTACTCGAGTAGATGAGAAACTGAACGTGG-3'

Other clones with same target sequence:

(none)