Construct: shRNA TRCN0000023000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007890.1-507s1c1
DNA Barcode:
CGGTATGAAATCGACTCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dyrk1a (13548)
Gene Description:
dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a
Transcript:
RefSeq NM_007890.1 (NON-CURRENT)
Match location:
Position 507 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTATGAAATCGACTCCTTA
Hairpin Sequence:
5'-CCGG-CGGTATGAAATCGACTCCTTA-CTCGAG-TAAGGAGTCGATTTCATACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTATGAAATCGACTCCTTACTCGAGTAAGGAGTCGATTTCATACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTATGAAATCGACTCCTTACTCGAGTAAGGAGTCGATTTCATACCG-3'

Other clones with same target sequence:

(none)