Construct: shRNA TRCN0000023040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007661.2-451s1c1
DNA Barcode:
GCCAGAGTGAAAGAGAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk11b (12537)
Gene Description:
cyclin-dependent kinase 11B
Transcript:
RefSeq NM_007661.2 (NON-CURRENT)
Match location:
Position 451 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAGTGAAAGAGAAAGAA
Hairpin Sequence:
5'-CCGG-GCCAGAGTGAAAGAGAAAGAA-CTCGAG-TTCTTTCTCTTTCACTCTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAGTGAAAGAGAAAGAACTCGAGTTCTTTCTCTTTCACTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAGTGAAAGAGAAAGAACTCGAGTTCTTTCTCTTTCACTCTGGC-3'

Other clones with same target sequence:

(none)