Construct: shRNA TRCN0000023084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009874.1-503s1c1
DNA Barcode:
CCAAACAACTTGTTGTTAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk7 (12572)
Gene Description:
cyclin-dependent kinase 7
Transcript:
RefSeq NM_009874.1 (NON-CURRENT)
Match location:
Position 503 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACAACTTGTTGTTAGAT
Hairpin Sequence:
5'-CCGG-CCAAACAACTTGTTGTTAGAT-CTCGAG-ATCTAACAACAAGTTGTTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACAACTTGTTGTTAGATCTCGAGATCTAACAACAAGTTGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACAACTTGTTGTTAGATCTCGAGATCTAACAACAAGTTGTTTGG-3'

Other clones with same target sequence:

(none)