Construct: shRNA TRCN0000023185

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011952.1-817s1c1
DNA Barcode:
AGGACCTTAATTGCATCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk3 (26417)
Gene Description:
mitogen-activated protein kinase 3
Transcript:
RefSeq NM_011952.1 (NON-CURRENT)
Match location:
Position 817 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000023185)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACCTTAATTGCATCATTA
Hairpin Sequence:
5'-CCGG-AGGACCTTAATTGCATCATTA-CTCGAG-TAATGATGCAATTAAGGTCCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACCTTAATTGCATCATTACTCGAGTAATGATGCAATTAAGGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACCTTAATTGCATCATTACTCGAGTAATGATGCAATTAAGGTCCT-3'

Other clones with same target sequence:

(none)